Article
  • Preparation and Characterization of Poly(ethylene glycol)-Doxorubicin/SPION Magnetic Nanoparticles for Cancer Therapy
  • Yugyeong Kim#, Seonmin Lee*,#, Danbee Kim*, Kangmin Noh, Keun Sang Oh, Sunghoon Cho**, Eunpyo Choi**,***, Kyu-pyo Kim*,† , and Kang Moo Huh

  • Dept. of Polymer Science and Engineering, Chungnam National University, 99 Daehakro, Yuseonggu, Daejeon 34134, Korea
    *Asan Medical center, Biomedical Research Center, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea
    **Medical Microrobot Center, Robot Research Initiative, Chonnam National University, Gwangju 61011, Korea
    ***Department of Mechanical Engineering, Chonnam National University, Gwangju 61186, Korea

  • 암 치료를 위한 Poly(ethylene glycol)-Doxorubicin/SPION 자성 나노입자의 제조 및 특성분석
  • 김유경# · 이선민*,# · 김단비* · 노강민 · 오근상 · 조성훈** · 최은표**,*** · 김규표*,† · 허강무

  • 충남대학교 고분자공학과, *서울아산병원 의생명연구소, **전남대학교 로봇연구소 마이크로의료로봇센터, ***전남대학교 기계공학부

Abstract

In this study, an amphiphilic polymer-drug conjugate was synthesized by chemical conjugation of a hydrophilic polymer poly(ethylene glycol) (PEG) with a hydrophobic anticancer agent, doxorubicin (DOX), to prepare magnetic nanoparticles for cancer diagnosis and therapy. The amphiphilic PEG-DOX polymer could self-assemble in the aqueous solution to form nanoparticles (NPs) and efficiently encapsulated superparamagnetic iron oxide nanoparticles (SPION) with high loading capacity and loading efficiency. The resulting SPION encapsulated PEG-DOX (PEG-DOX/SPION) NPs were observed to have an average size of ~100 nm with a narrow and unimodal size distribution and demonstrated the characteristic of MR contrast agent from the phantom experiments. In the toxicity assessment, PEG-DOX/SPION NPs showed lower toxicity than pristine DOX, and no mortality was detected at the maximum MTD. As a result, these magnetic NPs with high contents of DOX and SPION could be utilized as a theragnostic formulation for efficient cancer diagnosis and therapy, due to their diverse potential capacities properties, such as low toxicity, MR imaging character, passive and magnetic targeting properties, and so on.


Keywords: 본 연구에서는 친수성 poly(ethylene glycol)(PEG)와 소수성 항암제인 독소루비신(DOX)의 화학적 결합에 의해 합성된 PEG-DOX conjugate를 이용하여 암 진단 및 치료가 가능한 자성 나노입자를 제조하고자 하였다. 양친매성 PEG-DOX는 수용액 내에서 자기조립 특성을 보이고, 10 nm 내외의 자성 나노입자(SPION)에 대해 높은 봉입능 및 봉입률을 보였다. 제조된 PEG-DOX/SPION 나노입자는 ~100 nm의 균일한 사이즈 분포를 가지며 phantom 실험을 통해 조영제로서의 가능성을 확인하였다. 세포독성평가에서 DOX에 비해 낮은 독성을 보였고 최대내성용량 (MTD) 평가실험에서 사망 개체가 발생하지 않았다. 결과적으로, 고함량의 항암제와 SPION으로 구성된 본 나노입자 시스템은 낮은 독성, MR 조영 특성, 수동적 및 자성 표적화능 등 다양한 생기능성을 기반으로 암 진단 및 치료에 유용한 테라그노스틱 제형에 응용될 수 있을 것으로 기대한다.

Keywords: polymer-drug conjugate, PEG, doxorubicin, SPION, cancer diagnosis and therapy

  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2018; 42(6): 1059-1067

    Published online Nov 25, 2018

  • 10.7317/pk.2018.42.6.1059
  • Received on Jul 10, 2018
  • Revised on Aug 6, 2018
  • Accepted on Aug 7, 2018

Correspondence to

  • Kyu-pyo Kim*, and Kang Moo Huh
  • Dept. of Polymer Science and Engineering, Chungnam National University, 99 Daehakro, Yuseonggu, Daejeon 34134, Korea
    *Asan Medical center, Biomedical Research Center, 88, Olympic-ro 43-gil, Songpa-gu, Seoul 05505, Korea

  • E-mail: kkp1122@amc.seoul.kr, khuh@cnu.ac.kr