Chitosan have been often applied as medical polymer and L-lysine is a protein component of natural tissue. Artificial skin having good biocompatibility was prepared by the graft copolymerization of N^ε-carbobenzoxy-L-lysine NCA onto chitosan. Copolymerization was carried out with the conversion of about 70%. Synthesized copolymers were dissolved in dichloroacetic acid, however the decarbobenzoxylated ones had no good solvent. Their tensile strength and elongation under wet state showed 0.3∼0.5 kg/mm² and 10∼13%, respectively. Water vapor transmittance rate. however, was about 500 g/m²·day and the degree of biodegradation by protease was ca. 15%. Critical surface tension value was 30 dyn/cm. Biocompatibility was evaluated by studying human skin fibroblast cell culture on the prepared membrane surface. After 48 hours, cells were proliferated twice as many as initial cell density. In view of morphological changes, the distinctive features of fibroblast were observed well.

의료용재료로 응용되는 chitosan과 생체단백질구성 물질인 L-lysine온 공중합하여 생체적합성이 우수한 인공피부를 제조하였다. 공중합체의 합성시 70% 이상의 전화율을 보이며 합성한 copoly(chitosan-g-N^ε-carbobenzoxy-L-lysine)는 dichloroacetic arid에 용해 하였다. 그러나 공중합체중의 L-lysine잔기로부터 보호기를 제거하였을 경우에는 양용매를 찾을 수 없었다. 기계적 성질은 습윤시 인장강도가 0.3∼0.5 kg/mm². 신장율은 약 10%를 나타내었다. 수분투과도는 500 g/mm²·day의 값을 나타내었으며 단백질 분해효소에 의한 분해율은 15%/100ay 정도의 값을 보였다. 임계표면장력 값은 30 dyn/cm 였다. 결합조직 세포인 fibroblast를 사용하여 세포배양 실험을 한 결과 48시간 경과후에 control의 80% 이상, 초기 세포밀도의 2배 이상의 세포가 증식되었으며 세포의 모폴로지에서도 fibroblast의 특징적인 형태를 잘 관찰할 수 있었다.

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