Article
  • Fabrication and Characterization of Cell Spheroid Containing Porous Microparticles

  • Min Ji Kim, So Young Kim, Jeong Yun Kim*, and Se Heang Oh†

  • Department of Nanobiomedical Science, Dankook University, Cheonan 31116, Korea
    *Department of Physics, College of Science, Dankook University, Cheonan 31116, Korea

  • 다공성 미세입자가 포함된 세포 스페로이드의 제조 및 분석

  • 김민지 ·김소영 ·김정윤* ·오세행†

  • 단국대학교 나노바이오의과학과, *단국대학교 물리학과

  • Reproduction, stored in a retrieval system, or transmitted in any form of any part of this publication is permitted only by written permission from the Polymer Society of Korea.

References
  • 1. Rim, N. G.; Lee, Y. B.; Kim, S. J.; Shin, H. S. Current Status and Prospect of Biomaterials as Tissue Substitutes in Regenerative Medicine. Korean Ind. Chem. News. 2010, 13, 2-17.
  •  
  • 2. Mandrycky, C.; Phong, K.; Zheng, Y. Tissue Engineering Toward Organ-Specific Regeneration and Disease Modeling. MRS Commun. 2017, 7, 332-347.
  •  
  • 3. Registration status by registration institution wishing to donate and registration status of those wishing to donate by region. [Online], 2022, Center of Korean Network for Organ Sharing [KONOS] (Ministry of Health and Welfare). https://www.konos.go.kr/konosis/common/bizlogic.jsp (accessed January 14, 2022).
  •  
  • 4. Langer, R.; Vacanti, J. P. Tissue Engineering. Science 1993, 260, 920-926.
  •  
  • 5. Berthiaume, F.; Maguire, T. J.; Yarmush, M. L. Tissue Engineering and Regenerative Medicine: History, Progress, and Challenges. Annu. Rev. Chem. Biomol. Eng. 2011, 2, 403-430.
  •  
  • 6. Jin, H. R. Tissue Engineered Human Cartilage. Korean J. Otolaryngol. 2003, 46, 355-363.
  •  
  • 7. Shafiee, A.; Atala, A. Tissue Engineering: Toward a New Era of Medicine. Annu. Rev. Chem. Biomol. Eng. 2017, 68, 29-40.
  •  
  • 8. Thiemicke, A.; Jashnsaz, H.; Li, G.; Neuert, G. Generating Kinetic Environments to Study Dynamic Cellular Processes in Single Cells. Sci. Rep. 2019, 9, 1-10.
  •  
  • 9. Costa, E. C.; Moreira, A. F.; de Melo-Diogo, D.; Gaspar, V. M.; Carvalho, M. P.; Correia, I. J. 3D Tumor Spheroids: An Overview on the Tools and Techniques Used for Their Analysis. Biotechnol. Adv. 2016, 34, 1427-1441.
  •  
  • 10. Langhans, S. A. Three-dimensional In Vitro Cell Culture Models in Drug Discovery and Drug Repositioning. Front. Pharmacol. 2018, 9, 1-14.
  •  
  • 11. Sooriyaarachchi, D.; Minière, H. J.; Maharubin, S.; Tan, G. Z. Hybrid Additive Microfabrication Scaffold Incorporated with Highly Aligned Nanofibers for Musculoskeletal Tissues. Tissue Eng. Regen. Med. 2019, 16, 29-38.
  •  
  • 12. Fang, Y.; Eglen, R. M. Three-Dimensional Cell Cultures in Drug Discovery and Development. SLAS Discov. 2017, 22, 456-472.
  •  
  • 13. Cui, X.; Hartanto, Y.; Zhang, H. Advances in Multicellular Spheroids Formation. J. R. Soc. Interface 2017, 14, 20160877.
  •  
  • 14. Inch, W. R.; McCredie, J. A.; Sutherland, R. M. Growth of Nodular Carcinomas in Rodents Compared with Multi-Cell Spheroids in Tissue Culture. Growth 1970, 34, 271-282.
  •  
  • 15. Białkowska, K.; Komorowski, P.; Bryszewska, M.; Miłowska, K. Spheroids as a Type of Three-Dimensional Cell Cultures-Examples of Methods of Preparation and the Most Important Application. Int. J. Mol. Sci. 2020, 21, 6225.
  •  
  • 16. Ravi, M.; Paramesh, V.; Kaviya, S. R.; Anuradha, E.; Solomon, F. P. 3D Cell Culture Systems: Advantages and Applications. J. Cell Physiol. 2015, 230, 16-26.
  •  
  • 17. Liao, W.; Wang, J.; Xu, J.; You, F.; Pan, M.; Xu, X.; Weng, J.; Han, X.; Li, S.; Li, Y.; Liang, K.; Peng, Q.; Gao, Y. High-Throughput Three-Dimensional Spheroid Tumor Model Using a Novel Stamp-Like Tool. J. Tissue Eng. 2019, 10, 1-15.
  •  
  • 18. Ryu, N. E.; Lee, S. H.; Park, H. Spheroid Culture System Methods and Applications for Mesenchymal Stem Cells. Cells 2019, 8, 1620.
  •  
  • 19. Edmondson, R.; Broglie, J. J.; Adcock, A. F.; Yang, L. Three-Dimensional Cell Culture Systems and Their Applications in Drug Discovery and Cell-Based Biosensors. Assay Drug Dev. Techn. 2014, 12, 207-218.
  •  
  • 20. Park, J. H. Recent Advances in Three-Dimensional Multicellular Spheroid Formation for Biomedical Research. Korean J. Otorhinolaryngol-Head Neck Surg. 2020, 63, 245-251.
  •  
  • 21. Wang, A.; Madden, L. A.; Paunov, V. N. Advanced Biomedical Applications Based on Emerging 3D Cell Culturing Platforms. J. Mater. Chem. B 2020, 8, 10487-10501.
  •  
  • 22. Chaicharoenaudomrung, N.; Kunhorm, P.; Noisa, P. Three-Dimensional Cell Culture Systems as an In Vitro Platform for Cancer and Stem Cell Modeling. World J. Stem Cells 2019, 11, 1065-1083.
  •  
  • 23. Tseng, T. C.; Wong, C. W.; Hsieh, F. Y.; Hsu, S. H. Biomaterial Substrate‐Mediated Multicellular Spheroid Formation and Their Applications in Tissue Engineering. Biotechnol. J. 2017, 12, 1700064.
  •  
  • 24. Laschke, M. W.; Menger, M. D. Life is 3D: Boosting Spheroid Function for Tissue Engineering. Trends Biotechnol. 2017, 35, 133-144.
  •  
  • 25. Jeon, J. H.; Yun, B. G.; Lim, M. J.; Kim, S. J.; Lim, M. H.; Lim, J. Y.; Park, S. H.; Kim, S.W. Rapid Cartilage Regeneration of Spheroids Composed of Human Nasal Septum Derived Chondrocyte in Rat Osteochondral Defect Model. Tissue Eng. Regen. Med. 2020, 17, 81-90.
  •  
  • 26. Kim, J. H.; Lee, J. Y. Multi-Spheroid-Loaded Human Acellular Dermal Matrix Carrier Preserves Its Spheroid Shape and Improves In Vivo Adipose-Derived Stem Cell Delivery and Engraftment. Tissue Eng. Regen. Med. 2020, 17, 271-283.
  •  
  • 27. Curcio, E.; Salerno, S.; Barbieri, G.; De Bartolo, L.; Drioli, E. A. Bader, Mass Transfer and Metabolic Reactions in Hepatocyte Spheroids Cultured in Rotating Wall Gas Permeable Membrane System. Biomaterials 2007, 28, 5487-5497.
  •  
  • 28. Gilazieva, Z.; Ponomarev, A.; Rutland, C.; Rizvanov, A.; Solovyeva, V. Promising Applications of Tumor Spheroids and Organoids for Personalized Medicine. Cancers 2020, 12, 2727.
  •  
  • 29. Cesarz, Z.; Tamama, K. Spheroid Culture of Mesenchymal Stem Cells. Stem Cells Int. 2016, 2016, 9176357.
  •  
  • 30. Kojima, N.; Takeuchi, S.; Sakai, Y. Fabrication of Microchannel Networks in Multicellular Spheroids. Sensor. Actuat. B Chem. 2014, 198, 249-254.
  •  
  • 31. Lee, J.; Lee, S.; Ahmad, T.; Perikamana, S. K. M.; Lee, J.; Kim, E. M.; Shin, H. Human Adipose-Derived Stem Cell Spheroids Incorporating Platelet-Derived Growth Factor (PDGF) and Bio-minerals for Vascularized Bone Tissue Engineering. Biomaterials 2020, 255, 120192.
  •  
  • 32. Kim, H. Y.; An, B. S.; Kim, M. J.; Jeoung, Y. J.; Byun, J. H.; Lee, J. H.; Oh, S. H. Signaling Molecule-Immobilized Porous Particles with a Leaf-Stacked Structure as a Bioactive Filler System. ACS Biomater. Sci. Eng. 2020, 6, 2231-2239.
  •  
  • 33. Zhao, P.; Chen, L.; Shao, H.; Zhang, Y.; Sun, Y.; Ke, Y.; Ramakrishna, S.; He, L.; Xue, W. Cytotoxic and Adhesion-associated Response of NIH-3T3 Fibroblasts to COOH-Functionalized Multi-walled Carbon Nanotubes. Biomed. Mater. 2016, 11, 015021.
  •  
  • 34. Sun, X.; Kaufman, P. D. Ki-67: More than a Proliferation Marker. Chromosoma 2018, 127, 175-186.
  •  
  • 35. Kim, H. Y.; Lee, J. H.; Lee, H. A. R.; Park, J.-S.; Woo, D. K.; Lee, H.-C.; Rho, G.-J.; Byun, J.-H.; Oh, S. H. Sustained Release of BMP-2 from Porous Particles with Leaf-Stacked Structure for Bone Regeneration. ACS Appl. Mater. Interfaces 2018, 10, 21091-21102.
  •  
  • 36. Kim, M. J.; Lee, J. H.; Kim, J.-S., Kim, H. Y.; Lee, H.-C.; Byun, J.-H.; Lee, J.-H.; Kim, N.-H.; Oh, S. H. Intervertebral Disc Regeneration Using Stem Cell/Growth Factor-Loaded Porous Particles with a Leaf-Stacked Structure. Biomacromolecules 2020, 21, 4795-4805.
  •  
  • 37. Kuo, C. W.; Chueh, D. Y.; Chen, P. Investigation of Size Dependent Cell Adhesion on Nanostructured Interfaces. J. Nanobiotechnol. 2014, 12, 54.
  •  
  • 38. Park, S. G.; Li, M. X.; Eom, J. H.; Suh, E. Y.; Cho, W. K.; Joung, Y. K.; Huh, K. M. Synthesis and Characterization of Gallic Acid Conjugated Glycol Chitosans for Tissue Adhesive Applications. Polym. Korea 2021, 45, 372-379.
  •  
  • 39. Curcio, E.; Salerno, S.; Barbieri, G.; De Bartolo, L.; Drioli, E. A. Bader, Mass Transfer and Metabolic Reactions in Hepatocyte Spheroids Cultured in Rotating Wall Gas Permeable Membrane System. Biomaterials 2007, 28, 5487-5497.
  •  
  • 40. Lee, G. H.; Lee, J. S.; Oh, H. J.; Lee, S. H. Reproducible Construction of Surface Tension Mediated Honeycomb Concave Microwell Arrays for Engineering of 3D Microtissues with Minimal Cell Loss. PLoS One 2016, 11, e0161026.
  •  
  • 41. Weir, C.; Morel-Kopp, M.-C.; Gill, A.; Tinworth, K.; Ladd, L.; Hunyor, S. N.; Ward, C. Mesenchymal Stem Cells: Isolation, Characterisation and In Vivo Fluorescent Dye Tracking. Heart Lung Circ. 2008, 17, 395-403.
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2022; 46(3): 369-376

    Published online May 25, 2022

  • 10.7317/pk.2022.46.3.369
  • Received on Jan 25, 2022
  • Revised on Mar 4, 2022
  • Accepted on Mar 4, 2022

Correspondence to

  • Se Heang Oh

  • Department of Nanobiomedical Science, Dankook University, Cheonan 31116, Korea

  • E-mail: seheangoh@dankook.ac.kr