Article
  • Fabrication and Characterization of Porous PLLA Scaffolds with Gentamicin Sulfate Release System
  • Choi MG, Khang G, Lee IW, Rhee JM, Lee HB
  • 겐타마이신 설페이트를 서방화한 다공성 PLLA 지지체의 제조와 물성평가
  • 최명규, 강길선, 이일우, 이종문, 이해방
Abstract
PLLA scaffold loaded with gentamicin sulfate(GS) was prepared by emulsion freeze-drying method for the prevention of infection and the improvement of wettability, i.e., the cell-and tissue-compatibility. GS-loaded PLLA scaffolds were characterized by scanning electron microscopy(SEM), mercury porosimetry and blue dye intrusion, and the GS release pattern was analyzed by high performance liquid chromatography (HPLC). GS-loaded PLLA scaffolds with porosity above 30%, medium pore size ranging from 30 to 57 mum(with larger pore diameters greater than 150 mum), and specific pore area in the range of 35 to 75(m(2)/g) were manufactured by varying processing parameter as GS concentration. It was observed that GS-loaded PLLA scaffolds were highly porous with good interconnections between pores for allowing cell adhesion and growth. These scaffolds may be applicable for scaffold as structures that facilitate either tissue regeneration or repair during reconstructive operations.

본 연구에서는 세포 배양시 감염방지용으로 널리 쓰이는 겐타마이신 설페이트(GS) 가 서방화된 다공성 PLLA 지지체를 유화동결건조방법으로 제조하였다. 이들의 물성을 전자현미경 및 수은다공도계로 특성결정하였고, 적심성은 푸른염색 수용액으로 관찰하였으며, 방출거동은 high performance liquid chromatography (HPLC)를 이용하여 측정하였다. GS가 5, 10 및 20%가 포접된 PLLA 지지체의 다공도는 80∼90%이었으며 평균다공크기 범위는 30∼57㎛ 그리고 가장 큰 것으로는 150 ㎛이상의 것도 관찰되었다. 전체적인 다공도의 모양은 다공과 다공사이의 연결이 양호하고 대부분이 열린 셀 구조를 하고 있는 것으로 나타났다. 대조군에 비해 GS의 농도가 증가함에 따라 PLLA 지지체의 다공도가 감소하는 것으로 보아 GS내의 설페이트 부분은 친수성 역할 그리고 겐타마이신 부분은 소수성 역할을 수행하는 계면활성제의 역할에 기인한 것으로 사료된다. PLLA 지지체의 방출거동은 GS의 농도가 증가함에 따라 방출되는 양이 증가하였고, 적심성 또한 향상되어 세포배양시 긍정적 효과를 끼칠 것으로 예상되었다.

Keywords: PLLA Scaffold; Gentamicin Sulfate; Emulsion Freeze-Drying Method; Wetting Property

References
  • 1. Langer R, Vacanti JP, Science, 260, 920 (1993)
  •  
  • 2. Khang G, Lee JHThe Properties of Biomaterials, in Tissue Engineering: Concepts and Applications, eds. by J.J. Yoo and I. Lee, chap, 4. Korea Med. Pub. Co., Seoul (1998)
  •  
  • 3. Vacanti JP, Morse MA, Saltzman WM, Domb AJ, Perez-Atayde A, Langer R, J. Pediatr. Surg., 23, 3 (1998)
  •  
  • 4. Khang G, Lee HB, Chem. World, 37(3), 46 (1997)
  •  
  • 5. Khang G, Lee HB, J. Biomed. Eng. Res., 20(1), 1 (1999)
  •  
  • 6. Khang G, Jo I, Lee JH, Lee I, Lee HB, Polym. Sci. Technol., 10(5), 640 (1999)
  •  
  • 7. Khang G, Lee HB, Bioindustry, 22, 32 (1999)
  •  
  • 8. Khang G, Lee JH, Lee HB, Polym. Sci. Technol., 10(6), 732 (1999)
  •  
  • 9. Lee I, Khang G, Lee HB, Polym. Sci. Technol., 10(6), 782 (1999)
  •  
  • 10. Cima LG, Vacanti JP, Ingber D, Mooney D, Langer R, J. Biomech. Eng., 113, 143 (1991)
  •  
  • 11. Langer R, Cima LG, Tamada JA, Wintermantel E, Biomaterials, 11, 738 (1990)
  •  
  • 12. Holland SJ, Tighe BJ, Gould PL, J. Control. Release, 4, 155 (1986)
  •  
  • 13. Khang G, Jeon JH, Lee JW, Cho SC, Lee HB, Bio-Med. Mater. Eng., 7(6), 357 (1997)
  •  
  • 14. Khang G, Lee SJ, Lee JH, Kim YS, Lee HB, Bio-Med. Mater. Eng., 9(3), 179 (1999)
  •  
  • 15. Willians DF, Mort E, J. Bioeng., 1, 231 (1997)
  •  
  • 16. Bostaman O, J. Bone Joint Surg., 73A(1), 148 (1991)
  •  
  • 17. Singhal JP, Singh H, Ray AR, Macromol. Chem. Phys., C28, 475 (1988)
  •  
  • 18. Hollinger OJ, Schmitz JP, J. Oral Maxillofac. Surg., 45, 594 (1987)
  •  
  • 19. Khang G, Cho JC, Lee JW, Rhee JM, Lee HB, Bio-Med. Mater. Eng., 9(1), 49 (1999)
  •  
  • 20. Mooney DJ, Cima L, Langer R, Mater. Res. Soc. Symp. Proc., 252, 345 (1992)
  •  
  • 21. Klawitter J, Hulbert S, J. Biomed. Mater. Res. Symp., 2(1), 161 (1983)
  •  
  • 22. Yannas IV, Lee E, Orgill DP, Skrabat EM, Murghy GF, Proc. Nat'l Acad. Sci. USA, 86(3), 933 (1989)
  •  
  • 23. Spector M, Michon MJ, Smarook WH, Kwiatkowski GT, J. Biomed. Mater. Res., 12, 655 (1978)
  •  
  • 24. Mooney DJ, Breuer C, McNamara K, Vacanti JP, Langer R, Tissue Eng., 1(2), 107 (1995)
  •  
  • 25. Aubert JH, Clough RL, Polymer, 26, 2047 (1985)
  •  
  • 26. Gogleswski S, Pennings AJ, Macromol. Rapid Commun., 4, 675 (1983)
  •  
  • 27. Freed LE, Marquis JC, Nohria A, Emmanual J, Mikos AG, Langer R, J. Biomed. Mater. Res., 11, 27 (1993)
  •  
  • 28. Mooney DJ, Baldwin DF, Sub NP, Vacanti JP, Langer R, Biomaterials, 17, 1417 (1996)
  •  
  • 29. Khang G, Jeon JH, Cho JC, Lee HB, Polym.(Korea), 23(3), 471 (1999)
  •  
  • 30. Khang G, Jeon JH, Cho JC, Rhee JM, Lee HB, Polym.(Korea), 23(6), 861 (1999)
  •  
  • 31. Wald HL, Sarakinos G, Lyman MD, Mikos AG, Vacanti JP, Langer R, Biomaterials, 14, 270 (1993)
  •  
  • 32. Smith DM, Hua DW, Earl WL, MRS Bull., 19(4), 44 (1994)
  •  
  • 33. Mikos AG, Sarakinos G, Leite SM, Vacanti JP, Langer R, Biomaterials, 14(5), 323 (1993)
  •  
  • 34. Khang G, Lee SJ, Jeon JH, Lee JH, Lee HB, Polym.(Korea), 24(6), 869 (2000)
  •  
  • 35. Lee SJ, Khang G, Lee JH, Lee YM, Lee HB, Polym.(Korea), 24(6), 877 (2000)
  •  
  • 36. Ritter HL, Drake LC, Ind. Eng. Chem., 17, 782 (1945)
  •  
  • 37. Jamshidi K, Hyon SH, Ikada Y, Polymer, 29, 2229 (1988)
  •  
  • 38. Cho JC, Khang G, Rhee JM, Kim YS, Lee JS, Lee HB, Korea Polym. J., 7(2), 79 (1999)
  •  
  • 39. Khang G, Choi HS, Rhee JM, Yoon SC, Cho JC, Lee HB, Korea Polym. J., 8(6), 253 (2000)
  •  
  • 40. Choi HS, Kim SW, Yun DI, Khang G, Rhee JM, Kim YS, Lee HB, Polym.(Korea)to appear (2001)
  •  
  • 41. Son WI, Yun DI, Khang G, Kim BS, Kim YS, Lee HB, Biomater. Res.to appear (2001)
  •  
  • 42. Adamson AWPhysical Chemistry of Surfaces, p. 525, John Wiley, New York (1990)
  •  
  • 43. Everett DHBasic Principles of Colloid Science, p. 115, The Royal Society of Chemistry, London (1989)
  •  
  • 44. Schramm LL, Adv. Chem. Ser., 1, 231 (1992)
  •  
  • 45. Everett DHBasic Principles of Colloid Science, p. 130, The Royal Society of Chemistry, London (1989)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2001; 25(3): 318-326

    Published online May 25, 2001

  • Received on Jan 5, 2001