Article
  • Reduction of Inflammatory Reaction of PLGA Using Fibrin; in vivo Study
  • Kim SJ, Hong HH, Kim SH, Kim HL, Kim SH, Khang G
  • PLGA의 염증완화에 대한 피브린의 효과 ; in vivo 연구
  • 김수진, 홍현혜, 김순희, 김혜린, 김세호, 강길선
Abstract
In this study, we evaluated the effect of fibrin, a natural material, on the local inflammatory reaction of PLGA in vivo. PLGA degradation products can decrease the pH in the surrounding tissue, causing local inflammatory reaction. To solve this problem, fibrin/ PLGA scaffolds were implanted in 5-week-old Wister rats. To evaluate the influence of fibrin content on inflammatory cytokine expression induced by PLGA, RT-PCR analysis was used. Fibrous wall thickness and macrophage infiltration were evaluated by H&E and ED-1 immunohistochemical staining, respectively. In this study, we showed that fibrin/PLGA scaffolds reduced inflammatory reaction as compared to PLGA scaffold. We concluded that fibrin could reduce inflammatory response of PLGA.

PLGA는 미국 식품의약품안전청(FDA)의 승인을 받은 합성고분자로서 생체재료로 널리 쓰이고 있다. 하지만, 분해산물인 산으로 인하여 염증반응을 일으키고, 독성물을 생산하여 세포증식률의 감소를 야기시킨다고 보고된 바 있다. 이러한 PLGA의 단점을 보완하고자 생체재료인 피브린을 사용하였는데, 피브린은 피브리노겐의 풍부함과 이들의 정제가 비교적 용이하다는 장점을 가지고 있다. 본 연구에서는 PLGA의 염증완화에 대한 피브린의 효과를 알아보았다. 피브린 첨가에 따른 PLGA 다공성 지지체에 염증의 발현정도를 확인하기 위해 RT-PCR을 수행하였고 지지체와 조직 간의 상호작용을 통한 염증세포의 침윤과 대식세포 발생 정도를 확인하기 위해 H&E와 ED-1 염색을 수행하였다. 위 실험결과 천연재료인 피브린이 PLGA의 이물반응을 감소시키는데 긍정적인 영향을 미치는 것으로 확인되었다.

Keywords: fibrin; PLGA scaffold; inflammatory cytokine; in vivo

References
  • 1. Baek SH, Lee JH, Lee JW, Cho ML, Kim SI, Kim JI, Kim DS, Lee SH, Kim GT, J. Korean Rheum. Assoc., 15, 2 (2008)
  •  
  • 2. Klueh U, Dorsky DI, Moussy F, Kreutzer DL, J. Biomed. Mater. Res. A, 67, 838 (2003)
  •  
  • 3. Choi BS, Kim SH, Yoon SJ, Ha HJ, Kim MS, Yang YI, Son Y, Khang G, Rhee JM, Lee HB, Tissue. Eng. Reg. Med., 3, 295 (2006)
  •  
  • 4. Ko YK, Kim SH, Ha HJ, Kim MS, Han CW, Rhee JM, Son Y, Lee HB,. Khang G, Tissue. Eng. Regen. Med., 4, 67 (2007)
  •  
  • 5. Khang G, Kim MS, Min BY, Tissue. Eng. Regen. Med., 3, 376 (2004)
  •  
  • 6. Khang G, Kim MS, Cho SH, Tissue. Eng. Regen. Med., 1, 9 (2004)
  •  
  • 7. Kim SM, Kim SH, Kim CM, Oh AY, Kim GA, Lee IW, Rhee JM, Khang G, Kim MS, Lee HB, Tissue. Eng. Regen. Med., 4, 179 (2007)
  •  
  • 8. Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)
  •  
  • 9. Khang G, Lee SJ, Kim MS“Scaffolds; Tissue Engineering”, in Webser's Biomedical Engineering Handbook, 2nd Ed., Marcel Dekker, New York, Vol 1, 336 (2006)
  •  
  • 10. Jang JW, Lee B, Han CW, Kim MS, Cho SH, Lee HB, Khang G, Polym.(Korea), 28(5), 382 (2004)
  •  
  • 11. Taylor MS, Daniels AU, Andriano KP, Heller J, J. Appl. Biomater., 5, 151 (1994)
  •  
  • 12. Suganuma J, Alexander H, J. Appl. Biomater., 4, 13 (1993)
  •  
  • 13. Verheyen CC, Wijn JR, Blitterswijk CA, J. Biomed. Mater. Res., 27, 1115 (1993)
  •  
  • 14. Athanasiou K, Niederauer GG, Agrawal CM, Biomaterials, 17, 93 (1996)
  •  
  • 15. Anderson JM, Shive MS, Adv. Drug Del. Rev., 28, 5 (1997)
  •  
  • 16. Hong HH, Kim SH, Oh AY, Jeon NR, Jung SH, Lee SJ, Dyke M, Yoo JJ, Son Y, Rhee JM, Khang G, Tissue. Eng. Regen. Med., 5, 4 (2008)
  •  
  • 17. Kim SH, Lee D, Kang YS, Min BH, Son Y, Khang G, Tissue. Eng. Regen. Med., 6, 273 (2004)
  •  
  • 18. Kim SY, Tissue. Eng. Regen. Med., 5, 1 (2008)
  •  
  • 19. Oh AY, Kim SH, Kim SH, Lee IW, Kim MS, Rhee JM, Khang G, Lee HB, Tissue. Eng. Regen. Med., 4, 60 (2007)
  •  
  • 20. Lee EK, Lee JS, Park HS, Tissue. Eng. Regen. Med., 2, 94 (2005)
  •  
  • 21. Khang G, Lee SJ, Han CW, Rhee JM, Lee HB, Adv. Exp. Med. Biol., 534, 235 (2003)
  •  
  • 22. Lee KJ, Roper JG, Wang JC, Spine J., 5, 217 (2005)
  •  
  • 23. Lim JY, Kim SH, So JW, Choi JH, Rhee JM, Shin HS, Khang G, Tissue. Eng. Regen. Med., 5, 437 (2008)
  •  
  • 24. Shin YJ, Lee JK, Chung H, J. Korean Ophthalmol. Soc., 43, 4 (2002)
  •  
  • 25. Bae JS, Park MK, Park SW, Korean Chem. Eng., 47, 4 (2009)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2010; 34(1): 63-68

    Published online Jan 25, 2010

  • Received on Sep 16, 2009
  • Accepted on Oct 21, 2009