Article
  • Effect of Inflammatory Responses to PLGA Films Incorporated Hesperidin: In vitro and In vivo Results
  • Song JE, Shim CR, Lee Y, Ko HA, Yoon H, Lee D, Khang G
  • PLGA/헤스페리딘 함량별 필름에서 염증 완화 효과: In vitro, In vivo 결과
  • 송정은, 심초록, 이유정, 고현아, 윤현, 이동원, 강길선
Abstract
Hesperidin (Hes) has known to having some functions like protection of blood circulatory system, anti-tumor effect, antioxidant effect and anti-inflammatory effect. The goal of this study is to demonstrate the relationship between Hes and inflammatory through in vitro and in vivo studies using poly(lactic-co-glycolic acid) (PLGA) film including Hes as a tissue engineered scaffold. To confirm the proliferation of cells on fabricated scaffold, cells (RAW 264.7 and NIH/3T3) were seeded on PLGA/Hes film then analyzed with MTT and SEM at 1 and 3 days after seeding. The results from ELISA, RT-PCR, and FACS for anti-oxident and anti-inflammatory effect showed that inflammatory response of PLGA/Hes film decreased more than that of PLGA film. Also, in vivo result confirmed that inflammatory response by implanted PLGA/Hes film decreased more comparing with PLGA film. This is because of anti-inflammatory effect of Hes reducing induced inflammatory cell and accumulation of fibrous capsule. The results showed that PLGA/Hes film's capacity on reducing inflammatory is better than PLGA film because of Hes.

헤스페리딘은 혈관 순환계 보호 효과, 항암 효과, 항산화 효과, 항염증 효과 등의 작용이 있다고 알려져 있다. 본 연구에서는 헤스페리딘을 함유한 poly(lactic-co-glycolic acid)(PLGA) 필름을 조직공학적 세포담체로 이용하고 생체 내/외의 실험을 통하여 헤스페리딘과 염증 반응의 상관관계를 알아보고자 하였다. 세포담체에서의 세포의 증식과 부착을 확인하고자 PLGA/헤스페리딘 필름에 세포(RAW 264.7, NIH/3T3)를 파종하여 1, 3일 후에 MTT와 SEM을 통하여 분석하였다. 또한 항산화 효과와 염증 완화 효과를 알아보고자 ELISA, RT-PCR, FACS 등을 평가한 결과 PLGA 필름보다는 헤스페리딘이 첨가된 PLGA 필름에서 염증 반응이 감소하는 것을 확인하였다. In vivo 실험에서도 헤스페리딘이 PLGA에 의해 유도된 염증세포와 섬유피막 축적을 감소시켜 헤스페리딘의 항염증 효과를 볼 수 있었다. 이 실험 결과 PLGA 필름보다는 PLGA/헤스페리딘 필름에서 염증 반응이 감소함을 확인하였다.

Keywords: poly(lactic-co-glycolic acid); hesperidin; inflammatory response.

References
  • 1. Ko YK, Kim SH, Ha HJ, Kim MS, Han CW, Rhee JM, Son Y, Lee HB, Khang G, Tissue Eng. Regen. Med., 4, 67 (2007)
  •  
  • 2. Khang G, Kim MS, Min BY, Tissue Eng. Regen. Med., 3, 376 (2004)
  •  
  • 3. Khang G, Kim MS, Cho SH, Tissue Eng. Regen. Med., 1, 9 (2004)
  •  
  • 4. Yoon SJ, Kim SH, Ha HJ, Ko YK, So JW, Kim MS, Yang YI, Khang G, Rhee JM, Lee HB, Tissue Eng. Part A., 14, 539 (2008)
  •  
  • 5. Ko YK, Kim SH, Jeong JS, Ha HJ, Yoon SJ, Rhee JM, Kim MS, Lee HB, Khang G, Polym.(Korea), 31(1), 14 (2007)
  •  
  • 6. Song Y, Kwon J, Kim B, Jeon Y, Khang G, Lee D, J.Biomed. Mater. Res., 98A, 517 (2011)
  •  
  • 7. Oh SH, Kang SG, Lee JH, J. Mater. Sci. Mater. Med., 17, 131 (2006)
  •  
  • 8. Chan JM, Zhang L, Yuet KP, Liao G, Rhee JW, Langer R, Farokhzad OC, Biomaterials., 30, 1627 (2009)
  •  
  • 9. Bostman O, J. Bone Joint Surg. Am., 73, 148 (1991)
  •  
  • 10. Taylor MS, J. Appl. Biomater., 5, 151 (1994)
  •  
  • 11. Kim MS, Ahn HH, Shin YN, Cho MH, Khang G, Lee HB, Biomaterials., 28, 5137 (2007)
  •  
  • 12. Jeon YP, Mohammad LM, Park CH, Hong JH, Lee GD, Song JC, Kim GS, J. Life Science., 19, 479 (2009)
  •  
  • 13. Willoughby DA, Ann. Rheum. Dis., 34, 471 (1975)
  •  
  • 14. Cho EJ, Li L, Yamabe N, Kim HY, CNU J. Agricultural Science., 38, 717 (2011)
  •  
  • 15. Middleton E, Annal Aller., 61, 523 (1988)
  •  
  • 16. Lee Y, Howard LR, Villalon B, J. Food Sci., 60, 473 (1995)
  •  
  • 17. Marie HS, Joel L, Marin C, Toxicol. Appl. Pharmacol., 130, 73 (1995)
  •  
  • 18. Hong HH, Kim SJ, Kim SH, Kim HL, Park JH, Lee D, Khang G, Tissue Eng. Regen. Med., 6, 711 (2009)
  •  
  • 19. Sulaiman OAR, Gordon T, Glia., 32, 234 (2000)
  •  
  • 20. Shim CR, Lee YJ, Ko HA, Kim MJ, Bae JW, Lee LH, Song JE, Khang G, Int. J. Tissue Regen., 3, 50 (2012)
  •  
  • 21. Muschler GE, Nakamoto C, Griffith LG, J. Bone Joint Surg. Am., 86A, 1541 (2004)
  •  
  • 22. Amens BN, Shigenoga MK, Hagen TM, Proc. Natl. Acad. Sci., 90, 7915 (1993)
  •  
  • 23. Harman D, J. Gerontol., 2, 298 (1956)
  •  
  • 24. Lee Y, Kwon J, Khang G, Lee D, Tissue Eng. Part A., 18, 1967 (2012)
  •  
  • 25. Delgado AV, McManus AT, Chambers JP, Neuropeptides., 37, 355 (2003)
  •  
  • 26. Aggarwal BB, Nat. Rev. Immunol., 3, 745 (2003)
  •  
  • 27. Kim SJ, Hong HH, Kim SH, Kim HL, Kim SH, Khang G, Polymer(Korea)., 1, 63 (2010)
  •  
  • 28. Meinel L, Hofmann S, Karageorgiou V, Kirker-Head C, McCool J, Gronowicz G, Zichner L, Langer R, Vunjak-Novakovica G, Kaplan DL, Biomaterials., 26, 147 (2005)
  •  
  • 29. Sy JC, Seshadri G, Yang SC, Brown M, Oh T, Dikalov S, Murthy N, Davis ME, Nat. Mater., 7(11), 863 (2008)
  •  
  • 30. Chen M, Gu H, Ye Y, Lin B, Sun L, Deng W, Zhang J, Liu J, Food Chem. Toxicol., 48, 2980 (2010)
  •  
  • 31. Nandakumar N, Rengarajan T, Haribabu L, Perumal S, Balasubramanian P, Biomedicine & Preventive Nutrition., 1, 207 (2011)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2013; 37(3): 323-331

    Published online May 25, 2013

  • Received on Oct 22, 2012
  • Accepted on Jan 6, 2013