Article
  • Development of Hydrogel Containing Catechin for Wound Dressing
  • Kim J, Cho EB, Lee KY
  • 카테킨이 함유된 창상피복제용 하이드로젤의 개발
  • 김진, 조은비, 이기영
Abstract
Catechin (CTEC) is well-known as a very powerful antioxidant, containing the effects of anti-inflammation and skin wound healing. In this study, CTEC/β-cyclodextrin (β-CD) nanoparticles were incorporated into poly(vinyl alcohol) (PVA)/pectin (PT) hydrogel. The composite was designed for the induction of re-epithelializaton in skin wound. CTEC/β-CD nanoparticles were prepared by a molecular complex method. The size of the CTEC nanoparticles formed in the hydrogel was in the range of 250±17.5 nm. The incorporation efficiency of CTEC in the nanoparticles was 74%. The cumulative amounts of CTEC released from the hydrogel containing CTEC nanoparticles in the buffers of pH7.4 and 5.5 were 86.51±3.14% and 35.95±2.14% of total CTEC loaded in the hydrogel within 72 h, respectively. Also, in the wound healing test, the CTEC nanoparticles-loaded PVA/PT hydrogel showed faster healing of the wound made in rat dorsum than the CTEC gel.

Catechin(CTEC)은 천연항산화제로 알려져 있고 항염증에 효과적이며 피부 창상치료제로 응용되고 있다. 이 연구는 β-cyclodextrin(β-CD)으로 나노입자화한 CTEC을 poly(vinyl alcohol)(PVA)/pectin(PT) 조성의 하이드로젤에 첨가하였다. 피부상처의 재상피화를 위해 CTEC과 β-CD을 분자복합(molecular complex) 방법에 의해 나노입자 제조를 수행하였다. 하이드로젤에 첨가된 CTEC 나노입자는 250±17.5 nm이며, 입자 내 CTEC의 담지 효율은 처음 첨가량의 74%가 담지되었음을 확인하였다. CTEC는 하이드로젤 필름으로부터 pH7.4와 pH5.5 버퍼에서 72시간 동안 각각 86.51±3.14%와 35.95±2.14%의 방출을 보였다. 창상치료 실험에서 CTEC 나노입자가 적재된 PVA/PT 조성의 하이드로젤은 CTEC이 함유된 도포형 젤보다 빠른 치유력을 관찰할 수 있었다.

Keywords: catechin; herbal medicine; transdermal delivery; re-epithelialization.

References
  • 1. Poor M, Howard R, Hall I, Appleton I, Am. J. Pathol., 165, 299 (2004)
  •  
  • 2. Qin Y, Wang HW, Karuppanapandian T, Kim W, Chin. J.Traumatol., 13, 91 (2010)
  •  
  • 3. Lim HJ, Kim HT, Oh EJ, Choi JH, Ghim HD, Pyun DG, Lee SB, Chung DJ, Chung HY, Polym.(Korea), 34(4), 363 (2010)
  •  
  • 4. Yao NZ, Huang C, Jin DD, Orthop. Surg., 1, 231 (2009)
  •  
  • 5. Martinez YN, Pinuel L, Castro GR, Breccia JD, Appl. Biochem. Biotechnol., 167(5), 1421 (2012)
  •  
  • 6. Yan C, Xiu Z, Li X, Hao C, J. Mol. Graph. Model., 26, 420 (2007)
  •  
  • 7. Lee HS, Cheong JT, Park HJ, Shin TK, Kim JH, Lee JM, J. Vet. Clin., 22, 43 (2005)
  •  
  • 8. Hwang KT, Kweon OK, Woo HM, Kim DY, Nam TC, J. Vet. Sci., 39, 645 (1999)
  •  
  • 9. Bao M, Lou Y, J. Cardiovasc. Pharm., 48, 834 (2006)
  •  
  • 10. Kim H, Kawazoe T, Han DW, Wound Repair Regen., 16, 714 (2008)
  •  
  • 11. Gomathi K, Gopinath D, Ahmed MR, Jayakumar R, Biomaterials., 24, 2767 (2003)
  •  
  • 12. Chikkan K, Takahashi H, Clin. Dermatol., 13, 299 (1995)
  •  
  • 13. Hassan CM, Peppas NA, Adv. Polym. Sci., 153, 37 (2000)
  •  
  • 14. Chen YC, Yu SH, Tsai GJ, Tang DW, Mi FL, Peng YP, J. Agric. Food. Chem., 58, 6728 (2010)
  •  
  • 15. Hwang S, Lee J, Polym.(Korea), 35(4), 332 (2011)
  •  
  • 16. Lee MS, Yang JC, Kim SH, Song IB, Kim MS, Khang G, Lee HB, Polym.(Korea), 30(3), 259 (2006)
  •  
  • 17. Han SK, Choi SJ, “The Demand of Dressing”, in Advance in Wound Care, Yoo HJ, Kim JH, Park MH, Lee KK, Lee JH, Yoo YS, Han SK, Yushimize G,Editors, Korea Medical Book Publisher Co., Seoul, 167 (2003)
  •  
  • 18. Folkman J, Shing Y, J. Biol. Chem., 267, 10931 (1992)
  •  
  • 19. Kapoor M, Howard R, Hall I, Appleton I, Am. J. Pathol., 165, 299 (2004)
  •  
  • 20. Hwang MR, Kim JO, Lee JH, Kim YI, Kim JH, Chang SW, Jin SG, Kim JA, Han SS, Ku SK, Yong CS, Choi HG, AAPS Pharm. Sci. Tech., 11, 1092 (2010)
  •  
  • Polymer(Korea) 폴리머
  • Frequency : Bimonthly(odd)
    ISSN 0379-153X(Print)
    ISSN 2234-8077(Online)
    Abbr. Polym. Korea
  • 2022 Impact Factor : 0.4
  • Indexed in SCIE

This Article

  • 2013; 37(4): 462-469

    Published online Jul 25, 2013

  • Received on Jan 29, 2013
  • Accepted on Mar 15, 2013